Analysis of post-translational modifications by LC-MS/MS.
作者： Hannah Johnson , Claire E. Eyers
DOI: 10.1007/978-1-60761-780-8_5
关键词:
摘要: Post-translational modifications are highly dynamic and known to regulate many cellular processes. Both the site stoichiometry of modification a given protein sequence can have profound effects on regulation function. Thus, identification sites post-translational is crucial for fully deciphering biological roles any protein. The acute typically low makes characterization challenging. development analytical strategies aid selective enrichment these species paramount. Ongoing developments in mass spectrometry resulting increased speed sensitivity analysis mean that has become ideal tool qualitative quantitative modifications. This chapter provides an overview most popular LC-MS/MS-based modified peptides/proteins spectrometric workflows targeted toward specific post-translationally analytes.
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