The Behavior of Trichomonas vaginalis in Tissue Cultures: A Movie

摘要: The object in making this film was to show how Trichomonas vaginalis behaves among the cells of body. reaction body is a slow process and shown with lantern slides taken after have been exposed flagellates from 20 24 hours. actors are Dr. Trussell's (1940) strain T. which he Garth Johnson sent by air mail Iowa City. They were free bacteria cultured Johnson's medium at pH 5.4 5.8. tissue cultures made 8and 9-day-old chick embryo intestines, grown hanging drops Tyrode Locke Lewis solution (Hogue, 1937) enriched extract. This had 5.8 has optimum for vaginalis. We 1943) carefully controlled experiments that culture can live same medium. used intestines embryos because tissues obtained them more nearly like those found vagina where trichomonad lives. contained epithelium, mesothelium, fibroblasts sympathetic nerves. first part movie shows soon they introduced into drop culture. seen swimming freely over plates epithelium swarming along cut edges explanted there evidently some material being given off attracts them. Here sometimes form rosettes 30 individuals roll edge trichomonads. Two these picture. In two places entering lumen pieces intestinal tube. suggestive since often treatment infections, disappear, only reappear later date. It seems possible above observations may retreat fold or openings cervical glands. Another pictures trichomonads plexuses nerves without seeming stimulate injure Some settle down on means their axostyles whirl about as feed. Several attached nerve. other plate epithelial cells, either attaching settling sending out proboscis-like anterior structure containing flagella undulating membrane 1943). flagellated swung side feeds then suddenly withdrawn. Sometimes action repeated place