Physiology of a Pure Culture of Trichomonas vaginalis. I: Population in Relation to pH.

摘要: The effect of pH upon the growth Trichomonas vaginalis is probably related to problem clinical control organism in cases natural infection. This paper concerns extremes which will support vitro and optimum for multiplication bacteria-free culture.†1The culture medium used was Difco liver infusion agar slants overlaid with 5% human serum modified Ringer's solution sterilized by filtration.‡ both fluid adjusted N/1 HC1 buffered 0.25% sodium phosphate. incubation temperature 37.5 ± 1°C. determinations were made a glass electrode checked against two standard buffers.The organisms counted hemocytometer after 2, 4, 6 days incubation. Each count included 10 large squares, having total volume 1 cu mm. Six cultures examined at each except those where extraneous circumstances reduced number 5. Only minor shifts ...