Heterogeneity of the simian immunodeficiency virus (SIV) specific CD8(+) T-cell response in mucosal tissues during SIV primary infection.

摘要: Abstract Virus-specific CD8 + T cells play an important role in controlling viral replication during acute primary infection. At this early stage, mucosal tissues represent a major site of replication. Therefore, the presence functional virus-specific effector mucosa infection is key issue pathogenesis In order to evaluate extent response, six rhesus macaques were infected with simian immunodeficiency virus (SIV)mac251 and sacrificed on day 28 following The activity SIV-effector was evaluated by means γ-IFN ELISpot assay autologous expressing SIV env , gag pol nef genes as antigen-presenting cells. This evaluation performed PBMCs, spleen, peripheral lymph node, gut-associated node lamina propria lymphocytes isolated from different sites. parallel, cell-associated load quantified all these tissues. Five had SIV-specific PBMCs and/or nodes. However, macaques, only present seven sites out 24 tested (the duodenum, jejunum, ileum colon separately for each animal), whereas they detected corresponding addition, mean frequency γ-IFN-secreting 117 ± 228 per 10 6 vs. 958 1184 gut associated nodes ( P = 0.001). No overall correlation observed between T-cell load: among 17 which isolated, no specific 13 conclusion, data indicate that frequencies are low may be importance regard intense at stage.