Clinically adaptable polymer enables simultaneous spatial analysis of colonic tissues and biofilms.

摘要: Microbial influences on host cells depend upon the identities of microbes, their spatial localization, and responses they invoke specific cell populations. Multimodal analyses both microbes in a spatially resolved fashion would enable studies into these complex interactions native tissue environments, potentially clinical specimens. While techniques to preserve each microbial compartments have been used examine tissues separately, we endeavored develop approaches simultaneously analyze compartments. Herein, established an original method for mucus preservation using Poloxamer 407 (also known as Pluronic F-127), thermoreversible polymer with mucus-adhesive characteristics. We demonstrate that this approach can spatially-defined bi-layer colon bacterial communities within, compared marked absence when were processed traditional formalin-fixed paraffin-embedded (FFPE) pipelines. Additionally, antigens antibody staining preserved signal intensity 16S rRNA fluorescence situ hybridization (FISH) was enhanced poloxamer-fixed samples. This turn enabled us integrate multimodal analysis modified multiplex immunofluorescence (MxIF) protocol. Importantly, formulated polymerize cross-link at room temperature use workflows. These results suggest fixative formulation be integrated biospecimen collection pipelines simultaneous cells.