Spatial and temporal organization of replicating Escherichia coli chromosomes.

作者: Ivy F. Lau , Sergio R. Filipe , Britta Søballe , Ole-Andreas Økstad , Francois-Xavier Barre

DOI: 10.1046/J.1365-2958.2003.03640.X

关键词:

摘要: The positions of DNA regions close to the chromosome replication origin and terminus in growing cells Escherichia coli have been visualized simultaneously, using new widely applicable reagents. Furthermore, these with respect a factory-associated protein analysed. Time-lapse analysis has allowed fate origins, termini FtsZ ring be followed lineage-specific manner during formation microcolonies. These experiments reveal aspects E. cell cycle demonstrate that region is frequently located asymmetrically, on pole side mid-cell. This asymmetry could provide mechanism by which segregation FtsK, at division septum, can act directionally ensure septal free before completion division.

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