Bidirectional migration of SeqA-bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli.
作者： Sota Hiraga , Chiyome Ichinose , Toshinari Onogi , Hironori Niki , Mitsuyoshi Yamazoe
DOI: 10.1046/J.1365-2443.2000.00334.X
关键词:
摘要: Background We previously found that SeqA protein, which binds preferentially to newly replicated hemimethylated DNA, is localized as discrete fluorescent foci in Escherichia coli cells. A single focus, at midcell, separates into two and these migrate abruptly opposite directions. Results The present study shows (i) appearance of depends on continuous DNA replication, suggesting the represent clusters consisting (ii) a synchronous round focus midcell directions midway through replication from oriC terminus, (iii) but copies remain linked with each other for 40 min after 30 °C. Subsequently, separate gradually towards both borders nucleoid before cell division. Conclusions A cluster SeqA-bound segment bipolar fashion during progress prior separation sister copies.
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