Review of methodologies and a protocol for the Agrobacterium -mediated transformation of wheat
作者： Huw D Jones , Angela Doherty , Huixia Wu
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摘要: Since the first report of wheat transformation by Agrobacterium tumefaciens in 1997, various factors that influence T-DNA delivery and regeneration tissue culture have been further investigated modified. This paper reviews current methodology literature describing provides a complete protocol we developed used to produce over one hundred transgenic lines both spring winter varieties.
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