Factors influencing T-DNA transfer from Agrobacterium to precultured immature wheat embryos (Triticum aestivum L.).
作者： Murielle Uzé , Ingo Potrykus , Christof Sautter
DOI: 10.1007/BF03543569
关键词: Botany 、 Horticulture 、 Gene expression 、 Beta-glucuronidase 、 Agrobacterium tumefaciens 、 Inoculation 、 Marker gene 、 Agrobacterium 、 Biology 、 Reporter gene 、 Plant cell
摘要: We have studied several factors influencing the transfer of T-DNA from Agrobacterium to wheat cells. tumefaciens and A. rhizogenes strains were screened using immature zygotic embryos two varieties Bobwhite Lona (Triticum aestivum L.). Transient plant cells was shown a β-glucuronidase (gus)-intron marker gene. LBA9402 transferred efficiently cells, where it highly expressed. The efficiency influenced by cocultivation time, concentration preculture time isolated before inoculation.
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