Endothelial cell phospholipid distribution and phospholipase activity during acute and chronic hypoxia.

摘要: We have previously reported alterations in cyclooxygenase metabolism cultured aortic and pulmonary arterial endothelial cells exposed to acute chronic hypoxia. These depended on the duration degree of hypoxic exposure, vascular bed from which were derived, possibly availability arachidonic acid secondary modifications metabolic substrate, membrane phospholipids, and/or phospholipase activity. To investigate this last point further, we compared plasma phospholipid distribution activity both hypoxia, using two different precursors (acetic acid) three preparations (cell homogenates, partially purified membranes, highly membranes). found that exposure hypoxia has profound complicated effects cell composition these depend origin Furthermore, response purity preparation precursor used study metabolism. Finally, studies suggested phospholipids during occurred a greater extent compartments other than membranes well-recognized tolerance may be due, part, preservation integrity their