Glycerol Increases Content and Activity of Human Cytochrome P-4502E1 in a Transduced HepG2 Cell Line by Protein Stabilization
作者： Ming-Xue Yang , Arthur I. Cederbaum
DOI: 10.1111/J.1530-0277.1997.TB03770.X
关键词:
摘要: Glycerol is widely used to stabilize cytochrome P-450 and prevent its transformation P-420. The effect of glycerol on the content activity human P-4502E1 (CYP2E1) in a HepG2 cell line that stably constitutively expresses this was evaluated by immunoassays oxidation p-nitrophenol. Addition 100 200 mM culture medium resulted 2 1/2- 3-fold increase CYP2E1 microsomes isolated from cells. Increases could be observed within 4 8 hr after addition medium. had no b5 or activities NADPH-cytochrome reductase NADH-cytochrome reductase. Upon cycloheximide stop protein synthesis, decreased with apparent half-lives 6 hr, respectively. prevented loss activity. Labeling [35S]methionine, followed pulse-chase experiments cold methionine immunoprecipitation indicated half-life for approximately 3 hr. increased 11 Stabilization not additive synergistic ethanol 4-methylpyrazole, suggesting all three agents elevate similar type mechanism model. These results indicate can interact it against proteolytic degradation, increasing enzyme thereby elevating CYP2E1.
sci-hub.se 参考文章(36)
E. Eliasson, I. Johansson, M. Ingelman-Sundberg, Substrate-, hormone-, and cAMP-regulated cytochrome P450 degradation. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 87, pp. 3225- 3229 ,(1990) , 10.1073/PNAS.87.8.3225
Frederick H. Daniels, Edward F. Leonard, Stanley Cortell, Glucose and glycerol compared as osmotic agents for peritoneal dialysis Kidney International. ,vol. 25, pp. 20- 25 ,(1948) , 10.1038/KI.1984.3
Robert E. McGehee, Martin J.J. Ronis, Robert M. Cowherd, Magnus Ingelman-Sundberg, Thomas M. Badger, Characterization of cytochrome P450 2E1 induction in a rat hepatoma FGC-4 cell model by ethanol☆ Biochemical Pharmacology. ,vol. 48, pp. 1823- 1833 ,(1994) , 10.1016/0006-2952(94)90469-3
L CLEJAN, A CEDERBAUM, Oxidation of pyrazole by reconstituted systems containing cytochrome P-450 IIE1 Biochimica et Biophysica Acta. ,vol. 1034, pp. 233- 237 ,(1990) , 10.1016/0304-4165(90)90082-8
Chris J. Patten, Hiroyuki Ishizaki, Toshifumi Aoyama, Maojung Lee, Shu M. Ning, Wen Huang, Frank J. Gonzalez, C.S. Yang, Catalytic properties of the human cytochrome P450 2E1 produced by cDNA expression in mammalian cells. Archives of Biochemistry and Biophysics. ,vol. 299, pp. 163- 171 ,(1992) , 10.1016/0003-9861(92)90258-X
F. Peter Guengerich, Dong Hyun Kim, Masahiko Iwasaki, Role of human cytochrome P-450 IIE1 in the oxidation of many low molecular weight cancer suspects Chemical Research in Toxicology. ,vol. 4, pp. 168- 179 ,(1991) , 10.1021/TX00020A008
Andrei I. VOZNESENSKY, John B. SCHENKMAN, Inhibition of cytochrome-P450 reductase by polyols has an electrostatic nature. FEBS Journal. ,vol. 210, pp. 741- 746 ,(1992) , 10.1111/J.1432-1033.1992.TB17475.X
Yan Dai, Julia Rashba-Step, Arthur I. Cederbaum, Stable expression of human cytochrome P4502E1 in HepG2 cells: characterization of catalytic activities and production of reactive oxygen intermediates. Biochemistry. ,vol. 32, pp. 6928- 6937 ,(1993) , 10.1021/BI00078A017
Byung J. Song, Tamihide Matsunaga, James P. Hardwick, Sang S. Park, Richard L. Veech, Chung S. Yang, Harry V. Gelboin, Frank J. Gonzalez, Stabilization of cytochrome p450j messenger ribonucleic acid in the diabetic rat Molecular Endocrinology. ,vol. 1, pp. 542- 547 ,(1987) , 10.1210/MEND-1-8-542
Erik Eliasson, Inger Johansson, Magnus Ingelman-Sundberg, Ligand-dependent maintenance of ethanol-inducible cytochrome P-450 in primary rat hepatocyte cell cultures Biochemical and Biophysical Research Communications. ,vol. 150, pp. 436- 443 ,(1988) , 10.1016/0006-291X(88)90539-6