Mammalian epidermal growth factor stimulates G-protein activity in Trypanosoma brucei.

摘要: The life cycle of Trypanosoma brucei is tightly controlled within its mammalian and insect hosts. involvement host signals in this process becoming more apparent, but little yet known the mechanisms transduction these trypanosome. present study shows that three previously described trypanosome growth promoters, foetal calf serum, bombesin epidermal factor (EGF), elicit high-affinity guanosine triphosphate (GTP) hydrolysis activity membranes, indicating G-proteins are involved signals. In particular, GTP was found to increase significantly with increasing concentrations EGF over range 0–20 nM, normal physiological concentration tissues, further supporting view trypanosomes may be specifically using as a signal.