Neomycin and paromomycin inhibit 30S ribosomal subunit assembly in Staphylococcus aureus.

摘要: A number of different antibiotics that prevent translation by binding to the 50S ribosomal subunit bacterial cells have recently been shown also assembly this subunit. Antibacterial agents affecting 30S particle activities not examined extensively for effects on small formation. The aminoglycoside paromomycin and neomycin bind specifically inhibit translation. These drugs were in Staphylococcus aureus see whether they had a second inhibitory effect assembly. 3H-uridine pulse chase assay was used examine kinetics synthesis presence absence each antibiotic. formation inhibited both compounds. At 3 µg/mL antibiotic reduced rate 80% compared with control cells. Both showed concentration-dependent inhibition formation, lesser For neomycin, IC50 equal viable cell an 2 µg/mL. They protein values (2.5 1.25 µg/mL). This is demonstration subunit-specific