MicroRNA-222 promotes human non-small cell lung cancer H460 growth by targeting p27.

摘要: Two highly homologous microRNAs (miRNAs, miRs), miR-222 and miR-221, act as a cluster in cellular regulation. We have previously reported that miR-221 promoted the growth of human non-small cell lung cancer line H460. However, role regulating H460 is unclear. cells were transfected with mimics, inhibitors or their negative controls effects confirmed by Real-time quantitative reverse transcription polymerase chain reactions (qRT-PCRs). Cell viability was assessed Counting Kit-8 (CCK-8) while proliferation determined 5-Ethynyl-2'-deoxyuridine (EdU) assay. P27 P57, two putative targets miR-222, checked qRT-PCRs. found overexpression increased proliferative rate opposite obtained down-regulation miR-222. but not P57 identified potential target negatively regulated protein level. In summary, present study indicates likely targeting P27. Inhibition might be novel therapy for cancer.