Short wheat challenge is a reproducible in‐vivo assay to detect immune response to gluten

摘要: Summary It has been reported that interferon (IFN)-γ-secreting T cells reactive to gluten can be detected in the peripheral blood of individuals with treated coeliac disease (CD) after a short consumption wheat-containing food. By contrast, very little is known about reproducibility this in-vivo procedure same patient cohort which underwent two, or more, consumptions. Fourteen patients remission consumed wheat bread for 3 days; 13 second challenge wash-out 3–10 months on strict gluten-free diet. Immune reactivity was analysed by detecting IFN-γ before and 6 days commencing Gliadin-specific IFN-γ-secreting CD4+ increased significantly day first challenge. These resulted as prevalently human leucocyte antigen (HLA)-DQ restricted phenotype gut homing, suggested expression β7-integrin. Similarly, reactiveness gliadin observed consumption, although an individual variability responses at each Our findings confirmed non-invasive approach investigate gluten-related immune response subjects intolerant gluten. Furthermore, we demonstrated reproduced subject least months. study important implications application clinical practice.