Tolerance to O6-Methylguanine and 6-Thioguanine Cytotoxic Effects: A Cross-resistant Phenotype in N-Methylnitrosourea-resistant Chinese Hamster Ovary Cells
作者： E. Dogliotti , A. Zijno , A. Di Muccio , G. Aquilina , A. M. Giammarioli
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摘要: The biochemical and genetic characteristics of a clone Chinese hamster ovary cells displaying resistance to N-methyl-N-nitrosourea (MNU) 6-thioguanine (6-TG) were analyzed. initial level 7-methylguanine, 3-methyladenine, O6-methylguanine formation the repair rates for these alkylated bases same in resistant parental cell line, indicating that alkylation damage is not due differences DNA alkylation. After exposure 24 or 48 h 6-TG (0.6 micrograms/ml) culture medium, contrast them, was able replicate containing base analogue during following h. These data are agreement with hypothesis tolerate both present DNA. tolerance MNU also included chromosomal induced by two agents, MNU-resistant incurred less sister chromatid exchanges after treatment either 6-TG. 6-TG-resistant cells, selected growth 6-TG, exhibited cross-resistance but methyl methanesulfonate, confirming common pathway responsible O6-methylguanine.
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