[10] Thin-layer chromatography of neutral glycosphingolipids and gangliosides

摘要: Publisher Summary This chapter describes the thin-layer chromatography of neutral glycosphingolipids (GSLs) and gangliosides. GSLs are compounds composed ceramide (N-acylsphingosine) one or more sugars linked glycosidically to terminal hydroxyl group sphingosine. They occur in nature as constituents biological membranes lipoproteins, usually constitute a minor fraction lipid mixture. The resolution components mixture is much better when sample applied thin band rather than spot. Resorcinol spray reagent specific for containing sialic acid used detect most commonly developing solvents mixtures chloroform, methanol, water. segment plate glycolipid standard separated from with glass cutter, detected α-naphthol matched main individual bands marked scraped. chromatographic mobilities native gangliosides their labeled derivatives identical under systems generally used, which make this procedure useful identifying other purposes.