Spectrally-broad coherent anti-Stokes Raman scattering hyper-microscopy utilizing a Stokes supercontinuum pumped at 800 nm

摘要: We demonstrate spectral-focusing based coherent anti-Stokes Raman scattering (SF-CARS) hyper-microscopy capable of probing vibrational frequencies from 630 cm−1 to 3250 using a single Ti:Sapphire femtosecond laser operating at 800 nm, and commercially-available supercontinuum-generating fibre module. A broad Stokes supercontinuum with significant spectral power wavelengths between nm 940 is generated by tuning the module atypically long and/or chirped ~200 fs pump pulses, allowing convenient access lower in fingerprint region. This work significantly reduces instrumental technical requirements for multimodal CARS microscopy, while expanding capabilities an established approach SF-CARS.