Alr2954 of Anabaena sp. PCC 7120 with ADP-ribose pyrophosphatase activity bestows abiotic stress tolerance in Escherichia coli.
作者： Prashant Kumar Singh , Alok Kumar Shrivastava , Shilpi Singh , Ruchi Rai , Antra Chatterjee
DOI: 10.1007/S10142-016-0531-Y
关键词:
摘要: In silico derived properties on experimental validation revealed that hypothetical protein Alr2954 of Anabaena sp. PCC7120 is ADP-ribose pyrophosphatase, which belongs to nudix hydrolase superfamily. Presence binding site was attested by pyrophosphatase activity (K m 44.71 ± 8.043 mM, V max 7.128 ± 0.417 μmol min−1 mg protein−1, and K cat/K 9.438 × 104 μM−1 min−1). Besides ADP-ribose, the enzyme efficiently hydrolyzed various nucleoside phosphatases such as 8-oxo-dGDP, 8-oxo-dADP, 8-oxo-dGTP, 8-oxo-dATP, GDP-mannose, ADP-glucose, NADH. qRT-PCR analysis alr2954 showed significant expression under different abiotic stresses reconfirming its role in stress tolerance. Thus, qualifies be a member superfamily, serves assists multiple
springer.com
sci-hub.se 参考文章(72)
Alok Kumar Shrivastava, Antra Chatterjee, Shivam Yadav, Prashant Kumar Singh, Shilpi Singh, L.C. Rai, UV-B stress induced metabolic rearrangements explored with comparative proteomics in three Anabaena species. Journal of Proteomics. ,vol. 127, pp. 122- 133 ,(2015) , 10.1016/J.JPROT.2015.05.014
Prashant Kumar Singh, Alok Kumar Shrivastava, Antra Chatterjee, Sarita Pandey, Snigdha Rai, Shilpi Singh, L.C. Rai, Cadmium toxicity in diazotrophic Anabaena spp. adjudged by hasty up-accumulation of transporter and signaling and severe down-accumulation of nitrogen metabolism proteins Journal of Proteomics. ,vol. 127, pp. 134- 146 ,(2015) , 10.1016/J.JPROT.2015.05.019
David N. Frick, Maurice J. Bessman, Cloning, purification, and properties of a novel NADH pyrophosphatase. Evidence for a nucleotide pyrophosphatase catalytic domain in MutT-like enzymes. Journal of Biological Chemistry. ,vol. 270, pp. 1529- 1534 ,(1995) , 10.1074/JBC.270.4.1529
Sandra B. Gabelli, Mario A. Bianchet, Maurice J. Bessman, L. Mario Amzel, The structure of ADP-ribose pyrophosphatase reveals the structural basis for the versatility of the Nudix family. Nature Structural & Molecular Biology. ,vol. 8, pp. 467- 472 ,(2001) , 10.1038/87647
James A. Imlay, How oxygen damages microbes: oxygen tolerance and obligate anaerobiosis Advances in Microbial Physiology. ,vol. 46, pp. 111- 153 ,(2002) , 10.1016/S0065-2911(02)46003-1
S K Apte, A A Bhagwat, Salinity-stress-induced proteins in two nitrogen-fixing Anabaena strains differentially tolerant to salt. Journal of Bacteriology. ,vol. 171, pp. 909- 915 ,(1989) , 10.1128/JB.171.2.909-915.1989
Thermostability and aliphatic index of globular proteins. Journal of Biochemistry. ,vol. 88, pp. 1895- 1898 ,(1980) , 10.1093/OXFORDJOURNALS.JBCHEM.A133168
Akiyoshi Higo, Masahiko Ikeuchi, Masayuki Ohmori, cAMP regulates respiration and oxidative stress during rehydration in Anabaena
sp. PCC 7120 FEBS Letters. ,vol. 582, pp. 1883- 1888 ,(2008) , 10.1016/J.FEBSLET.2008.05.007
RomanA. Laskowski, J.AntoonC. Rullmann, MalcolmW. MacArthur, Robert Kaptein, JanetM. Thornton, AQUA and PROCHECK-NMR: Programs for checking the quality of protein structures solved by NMR Journal of Biomolecular NMR. ,vol. 8, pp. 477- 486 ,(1996) , 10.1007/BF00228148
David N. Frick, Benjamin D. Townsend, Maurice J. Bessman, A Novel GDP-Mannose Mannosyl Hydrolase Shares Homology with the MutT Family of Enzymes Journal of Biological Chemistry. ,vol. 270, pp. 24086- 24091 ,(1995) , 10.1074/JBC.270.41.24086