Interaction of melittin with endotoxic lipid A.
作者： S.A. David , V.I. Mathan , P. Balaram
DOI: 10.1016/0005-2760(92)90006-H
关键词:
摘要: Several amphipathic and cationic substances are known to bind lipid A, the toxic component of bacterial lipopolysaccharides. In this report, we have characterized, by fluorescence methods, interaction melittin, an basic 26-residue polypeptide isolated from bee venom, with A. The stoichiometry complex appears be two molecules melittin one A a dissociation constant 2.5 × 10 −6 M. binding not only modifies endotoxic properties in number biological assays, but also results abrogation hemolytic activity melittin. model is proposed based on structures observed binding.
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sci-hub.se 参考文章(36)
Burton J. Litman, Y. Barenholz, [91] Fluorescent probe: Diphenylhexatriene Methods in Enzymology. ,vol. 81, pp. 678- 685 ,(1982) , 10.1016/S0076-6879(82)81093-8
D Rifkind, Prevention by polymyxin B of endotoxin lethality in mice. Journal of Bacteriology. ,vol. 93, pp. 1463- 1464 ,(1967) , 10.1128/JB.93.4.1463-1464.1967
D C Morrison, S Raziuddin, Binding of bacterial endotoxin (LPS) to encephalitogenic myelin basic protein and modulation of characteristic biologic activities of LPS. Journal of Immunology. ,vol. 126, pp. 1030- 1035 ,(1981)
Meir Shinitzky, Yechezkel Barenholz, Dynamics of the Hydrocarbon Layer in Liposomes of Lecithin and Sphingomyelin Containing Dicetylphosphate Journal of Biological Chemistry. ,vol. 249, pp. 2652- 2657 ,(1974) , 10.1016/S0021-9258(19)42780-4
D. Anderson, T.C. Terwilliger, W. Wickner, D. Eisenberg, Melittin forms crystals which are suitable for high resolution X-ray structural analysis and which reveal a molecular 2-fold axis of symmetry. Journal of Biological Chemistry. ,vol. 255, pp. 2578- 2582 ,(1980) , 10.1016/S0021-9258(19)85931-8
Harald LABISCHINSKI, Eberhard VORGEL, Willi UEBACH, Roland Peter MAY, Hans BRADACZEK, Architecture of bacterial lipid A in solution. A neutron small-angle scattering study. FEBS Journal. ,vol. 190, pp. 359- 363 ,(1990) , 10.1111/J.1432-1033.1990.TB15583.X
Otto Lüderitz, Marina A. Freudenberg, Chris Galanos, Volker Lehmann, Ernst Th. Rietschel, Derek H. Shaw, Lipopolysaccharides of Gram-Negative Bacteria Current topics in membranes and transport. ,vol. 17, pp. 79- 151 ,(1982) , 10.1016/S0070-2161(08)60309-3
S Kotani, H Takada, I Takahashi, M Tsujimoto, T Ogawa, T Ikeda, K Harada, H Okamura, T Tamura, S Tanaka, Low endotoxic activities of synthetic Salmonella-type lipid A with an additional acyloxyacyl group on the 2-amino group of beta (1-6) glucosamine disaccharide 1,4'-bisphosphate. Infection and Immunity. ,vol. 52, pp. 872- 884 ,(1986) , 10.1128/IAI.52.3.872-884.1986
J G Sawyer, N L Martin, R E Hancock, Interaction of macrophage cationic proteins with the outer membrane of Pseudomonas aeruginosa. Infection and Immunity. ,vol. 56, pp. 693- 698 ,(1988) , 10.1128/IAI.56.3.693-698.1988
James J Corrigan, James F Kiernat, Effect of polymyxin B sulfate on endotoxin activity in a gram-negative septicemia model. Pediatric Research. ,vol. 13, pp. 48- 51 ,(1979) , 10.1203/00006450-197901000-00011