Rapid Axillary Bud Proliferation and ex vitro Rooting of Eupatorium triplinerve
作者： K.P. Martin
DOI: 10.1023/B:BIOP.0000041067.23890.58
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摘要: Effective protocol was established for micropropagation of the medicinal plant Eupatorium triplinerve Vahl through rapid axillary bud proliferation and ex vitro rooting. Murashige Skoog (MS) medium fortified with 8.87 μM benzylaminopurine (BAP) 2.46 indole-3-butyric acid (IBA) best developed a mean 8.1 shoots per node. Excision culture node segments in on supplemented same concentration growth regulators more than 30 within 40 d. Shoot multiplication did not exhibit decrease number even at 7th subculture. Dipping basal end IBA solution 10 d induced roots its transfer to small pots facilitated survival all rooted (100 %). Ex rooting by direct from showed 92 % survival.
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