High frequency and nonrandom distribution of alloreactivity in T cell clones selected for recognition of foreign antigen in association with self class II molecules.

摘要: Previous studies have demonstrated that a single T cell clone can respond to both foreign antigen in the context of self major histocompatibility complex (MHC)-encoded molecules (self plus X) and an allogeneic class I or II molecule absence (non-self). We used limiting dilution cells obtained from draining lymph nodes antigen-primed B10.A mice establish large number clones recognize either GAT, pigeon cytochrome c, sheep insulin association with syngeneic antigen-presenting cells. Sixty-two antigen-specific were assayed for their ability proliferate response panel nine different haplotypes. Of these, 38 (61%) responded at least one haplotype, 15 (39%) more than stimulator. In addition, patterns alloreactivity varied immunizing antigen. The GAT-specific had responder every haplotype tested, although H-2u-responsive most common. contrast, no c-specific stimulators H-2u but rather predominantly H-2t4/H-2s H-2i5/H-2b. Finally, insulin-reactive preferentially stimulators, stimulation by H-2p H-2q haplotypes was also A chi 2 analysis data dependence pattern observed upon immunization statistically significant (p less 0.01). high frequency found is discussed, as well implications antigen-dependent skewing distribution one-receptor model vs two-receptor recognition.