Fibrotic scar model and TGF-β1 differently modulate action potential firing and voltage-dependent ion currents in hippocampal neurons in primary culture.

摘要: Traumatic injury of the central nervous system is accompanied by various functional and morphological changes. Animal models traumatic brain are commonly used to investigate changes in behaviour, morphology, expression proteins around site injury, or diagnostically important biomarkers. Excitability a single neuron at, close to, was rarely investigated. Several in vitro were developed which allow such investigation. In present work, we employed fibrotic scar model according Kimura-Kuroda coauthors analyse altered excitability rat hippocampal neurons under conditions mimicking injury. Hippocampal from newborn rats cultured either on presence TGF-β1, cytokine secreted at that may have both neuroprotective neurodegenerative function. Fibrotic facilitated ability neonatal fire action potential series increasing density voltage activated sodium potassium currents. Chondroitin sulphate proteoglycans played substantial role these effects, as proven their full reversion after administration Chondroitinase ABC. contrast, TGF-β1 did not contribute them. An application itself attenuated generation potentials, inhibited current potentiated Main alteration electrophysiological parameters caused enhanced excitability. predominantly injured hippocampus.