Conversion of arachidonic acid to two novel products by a cytochrome P450-dependent mixed-function oxidase in polymorphonuclear leukocytes.

摘要: Abstract Canine polymorphonuclear leukocytes metabolize [14C] arachidonic acid into 2 unidentified products, separated by thin-layer chromatography and high performance liquid chromatography, called peak 1 2. The formation of is maximal at 5 minutes then declines, while the synthesis increases throughout 30 minute incubation period. and, to a lesser extent, 2, was enhanced after dual inhibition lipoxygenase cyclo-oxygenase enzymes with BW755C (94μm) or nafazatrom (37μm), in calcium-free buffer. In contrast, these products inhibited SKF-525A (50μm), suggesting cytochrome P450-dependent mechanism. presence P450 neutrophil microsomes confirmed measuring aryl hydrocarbon hydroxylase activity content.