A comparative study of mammalian and reptilian alternative pathway of complement-mediated killing of the Lyme disease spirochete (Borrelia burgdorferi).

摘要: The potential bactericidal activity of the alternative complement pathway mammalian and reptilian sera to Borrelia burgdorferi sensu stricto (s.s.) was evaluated in vitro. Complement-mediated killing observed when cultured spirochetes were inoculated into from western fence lizard (Sceloporus occidentalis) southern alligator (Elgaria multicarinata), but not they serum either deer mouse (Peromyscus maniculatus) or humans. Spirochetes still alive after 4 hr that had been preheated at 56 C for 30 min inactivate complement. Furthermore, chelated with 10 mM ethylenediaminetetraacetic acid block all activation, borreliacidal arrested. When ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic plus MgCl2 only classical >85% immobilized within 1 hr. Diff...