A Validated LC-MS/MS Method for Simultaneous Determination of Six Aconitum Alkaloids and Seven Ginsenosides in Rat Plasma and Application to Pharmacokinetics of Shen-Fu Prescription.
作者： Huizi Ouyang , Fang Liu , Zhidan Tang , Xiaopeng Chen , Fang Bo
DOI: 10.1155/2018/5107083
关键词:
摘要: A sensitive and reliable LC-MS/MS method has been developed validated for simultaneous determination of six Aconitum alkaloids (aconitine, hypaconitine, mesaconitine, benzoylaconitine, benzoylhypacoitine, benzoylmesaconine) seven ginsenosides (Rb1, Rb2, Rc, Rd, Re, Rf, Rg1) in rat plasma after oral administration Shen-Fu prescription. Psoralen was selected as internal standard (IS). Protein precipitation with methanol used sample preparation. The chromatographic separation achieved on a CORTECS™ C18 column 0.1% formic acid aqueous solution acetonitrile mobile phase. flow rate 0.3 mL/min. detection performed tandem mass system an electrospray ionization (ESI) source the positive multiple-reaction monitoring (MRM) mode. calibration curves were linear over range 0.1-50 1-500 ng/mL, respectively. extraction recoveries analytes samples ranged from 64.2 to 94.1%. Meanwhile, intra- interday precision less than 14.3%, accuracy −14.2% 9.8%. successfully applied pharmacokinetics
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