Human T-cell clones against Toxoplasma gondii : production of interferon-γ, interleukin-2, and strain cross-reactivity
作者： P. H�rion , R. Saavedra
DOI: 10.1007/BF00931632
关键词:
摘要: The soluble fraction from a sonicate ofToxoplasma gondii tachyzoites (termed F3) was shown to induce dose-dependent blastic transformation of peripheral-blood mononuclear cells (PBMC) seropositive individuals only and used isolate panel T-cell clones the PBMC an immune donor. Proliferation assays using F3 showed that 15 (14 CD4+ 1 CD8+) 18 isolated were specific forT. gondii. In response antigen stimulation, 5 produced detectable levels interleukin-2 (IL-2, 0.2–15 u/ml) 9 significant interferon-γ (IFN-γ, 17.5–1400 IU/ml). Seven 7 tested reacted with two differentToxoplasma strains (RH Wiktor). When as antigen-presenting cells, autologous B-lymphoblastoid cell line could efficiently present three six tested. This study identifies characterizes cellular probes be useful for future vaccine design.
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sci-hub.se 参考文章(27)
H. P. A. Hughes, Toxoplasmosis: the need for improved diagnostic techniques and accurate risk assessment. Current Topics in Microbiology and Immunology. ,vol. 120, pp. 105- 139 ,(1985) , 10.1007/978-3-662-09197-5_6
Mary M. Ferm, Steven Gillis, Winny Ou, Kendall A. Smith, T Cell Growth Factor: Parameters of Production and a Quantitative Microassay for Activity Journal of Immunology. ,vol. 120, pp. 2027- 2032 ,(1978)
A Lanzavecchia, M Pletscher, R Scheper, F Sinigaglia, G Garotta, D Scheidegger, Isolation and characterization of Ni-specific T cell clones from patients with Ni-contact dermatitis. Journal of Immunology. ,vol. 135, pp. 3929- 3932 ,(1985)
J. K. Frenkel, Adoptive Immunity to Intracellular Infection Journal of Immunology. ,vol. 98, pp. 1309- 1319 ,(1967)
M Chinchilla, J K Frenkel, Specific mediation of cellular immunity to Toxoplasma gondii in somatic cells of mice. Infection and Immunity. ,vol. 46, pp. 862- 866 ,(1984) , 10.1128/IAI.46.3.862-866.1984
Y Suzuki, J S Remington, Dual regulation of resistance against Toxoplasma gondii infection by Lyt-2+ and Lyt-1+, L3T4+ T cells in mice. Journal of Immunology. ,vol. 140, pp. 3943- 3946 ,(1988)
S D Sharma, J S Remington, J M Hofflin, In vivo recombinant interleukin 2 administration enhances survival against a lethal challenge with Toxoplasma gondii. Journal of Immunology. ,vol. 135, pp. 4160- 4163 ,(1985)
J S Remington, Y Suzuki, The effect of anti-IFN-gamma antibody on the protective effect of Lyt-2+ immune T cells against toxoplasmosis in mice. Journal of Immunology. ,vol. 144, pp. 1954- 1956 ,(1990)
M Ferrarini, V Pistoia, G Melioli, A Merli, A Terragna, S Roncella, A Canessa, An in vitro model for Toxoplasma infection in man. Interaction between CD4+ monoclonal T cells and macrophages results in killing of trophozoites. Journal of Immunology. ,vol. 140, pp. 3580- 3588 ,(1988)
E. R. Pfefferkorn, Interferon gamma blocks the growth of Toxoplasma gondii in human fibroblasts by inducing the host cells to degrade tryptophan. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 908- 912 ,(1984) , 10.1073/PNAS.81.3.908