Development and Histopathological Characterization of Tumorgraft Models of Pancreatic Ductal Adenocarcinoma

摘要: Pancreatic cancer is the one of deadliest all malignancies. The five year survival rate for patients with this disease 3-5%. Thus, there a compelling need novel therapeutic strategies to improve clinical outcome pancreatic cancer.  Several groups have demonstrated other types solid tumors that early passage human tumor xenograft models can be used define some genetic and molecular characteristics specific tumors. Published studies also suggest murine tumorgraft (early xenografts derived from direct implantation primary specimens) may useful in identifying compounds efficacy against types.  Because fatal few well-characterized model systems are available translational research, we developed characterized panel biological evaluation drug testing.  Of 41 specimens implanted subcutaneously into mice, 35 produced viable models.  We document fidelity histological morphological KRAS mutation status among (F0), F1, F2 twenty progressed F3 generation.  Importantly, our procedures take 85%, higher than any reported literature. Primary failed produce tumorgrafts were those either contained <10% cells or obtained significantly smaller In view through at least generation propose these represent tool critical evaluating potential therapies.