INT Toxicity over Natural Bacterial Assemblages from Surface Oligotrophic Waters: Implications for the Assessment of Respiratory Activity.

作者: Isabel Baños , María F. Montero , Mar Benavides , Javier Arístegui

DOI: 10.1007/S00248-019-01479-4

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摘要: Plankton community respiration (R) is a major component of the carbon flux in aquatic ecosystems. However, current methods to measure actual from oxygen consumption at relevant spatial scales are not sensitive enough oligotrophic environments where rates very low. To overcome this drawback, more indirect enzymatic approaches commonly used as R proxies. The vivo electron transport system (ETSvivo) assay, which measures reduction (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride salt, INT) INT-formazan presence natural substrate levels, was recently proposed an reliable estimation for plankton communities. under conditions, formazan salts could be toxic cells. Here, we test toxicity 0.2 mM final INT concentration, widely ETSvivo assays, on bacterial assemblages collected coastal and oceanic waters off Gran Canaria (Canary Islands, subtropical North Atlantic), eight independent experiments. After 0.5 h incubation, significant but variable decline cell viability (14–49%) observed all samples inoculated with INT. Moreover, also inhibited leucine uptake less than 90 min incubation. In light these results, argue that respiratory obtained method need interpreted caution derive regions bacteria largely contribute respiration. our experiments questions use single R/ETSvivo relationship universal proxy regional studies.

参考文章(23)
Javier Arístegui, Susana Agustí, Jack J. Middelburg, Carlos M. Duarte, Respiration in the mesopelagic and bathypelagic zones of the oceans Respiration in Aquatic Ecosystems/ Paul A. del Giorgio and Peter J. le B. Williams,(eds), cap. 10 p. 181-205. pp. 181- 205 ,(2005) , 10.1093/ACPROF:OSO/9780198527084.003.0010
G G Rodriguez, D Phipps, K Ishiguro, H F Ridgway, Use of a fluorescent redox probe for direct visualization of actively respiring bacteria. Applied and Environmental Microbiology. ,vol. 58, pp. 1801- 1808 ,(1992) , 10.1128/AEM.58.6.1801-1808.1992
Sandra Martínez-García, Emilio Fernández, María Aranguren-Gassis, Eva Teira, In vivo electron transport system activity: a method to estimate respiration in natural marine microbial planktonic communities Limnology and Oceanography-methods. ,vol. 7, pp. 459- 469 ,(2009) , 10.4319/LOM.2009.7.459
P. S. May, J. W. Winter, G. H. Fried, W. Antopol, Effect of tetrazolium salts on selected bacterial species. Experimental Biology and Medicine. ,vol. 105, pp. 364- 366 ,(1960) , 10.3181/00379727-105-26112
Javier Arístegui, María F. Montero, The relationship between community respiration and ETS activity in the ocean Journal of Plankton Research. ,vol. 17, pp. 1563- 1571 ,(1995) , 10.1093/PLANKT/17.7.1563
Paul B Hatzinger, Patrick Palmer, Richard L Smith, Cecilia T Peñarrieta, Tadashi Yoshinari, Applicability of tetrazolium salts for the measurement of respiratory activity and viability of groundwater bacteria. Journal of Microbiological Methods. ,vol. 52, pp. 47- 58 ,(2003) , 10.1016/S0167-7012(02)00132-X
P Servais, EO Casamayor, C Courties, P Catala, N Parthuisot, P Lebaron, Activity and diversity of bacterial cells with high and low nucleic acid content Aquatic Microbial Ecology. ,vol. 33, pp. 41- 51 ,(2003) , 10.3354/AME033041
Bruce J. Tuovila, Paul A. LaRock, Effect of species difference and growth rate in the use of INT as an indicator of bacterial respiration Journal of Microbiological Methods. ,vol. 4, pp. 185- 188 ,(1985) , 10.1016/0167-7012(85)90035-1