作者: Isabel Baños , María F. Montero , Mar Benavides , Javier Arístegui
DOI: 10.1007/S00248-019-01479-4
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摘要: Plankton community respiration (R) is a major component of the carbon flux in aquatic ecosystems. However, current methods to measure actual from oxygen consumption at relevant spatial scales are not sensitive enough oligotrophic environments where rates very low. To overcome this drawback, more indirect enzymatic approaches commonly used as R proxies. The vivo electron transport system (ETSvivo) assay, which measures reduction (2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl tetrazolium chloride salt, INT) INT-formazan presence natural substrate levels, was recently proposed an reliable estimation for plankton communities. under conditions, formazan salts could be toxic cells. Here, we test toxicity 0.2 mM final INT concentration, widely ETSvivo assays, on bacterial assemblages collected coastal and oceanic waters off Gran Canaria (Canary Islands, subtropical North Atlantic), eight independent experiments. After 0.5 h incubation, significant but variable decline cell viability (14–49%) observed all samples inoculated with INT. Moreover, also inhibited leucine uptake less than 90 min incubation. In light these results, argue that respiratory obtained method need interpreted caution derive regions bacteria largely contribute respiration. our experiments questions use single R/ETSvivo relationship universal proxy regional studies.