Interrelationships of endoplasmic reticulum, mitochondria, intermediate filaments, and microtubules--a quadruple fluorescence labeling study.

摘要: To study the interrelationships of endoplasmic reticulum, mitochondria, intermediate filaments, and microtubules, we have developed a quadruple fluorescence labeling procedure to visualize all four structures in same cell. We applied this approach cellular organization control cells treated with microtubule drugs vinblastine or taxol. Endoplasmic reticulum was visualized by staining glutaraldehyde-fixed dye 3,3'-dihexyloxacarbocyanine iodide. After detergent permeabilization, triple immunofluorescence carried out specifically vimentin microtubules. Mitochondria human fibroblasts were found be highly elongated tubular (lengths up greater than 50 microns), which many cases apparently fused each other. always observed associated although also existed independently. Intermediate filament distribution could not completely account for mitochondrial distributions. Microtubules, however, codistributed these organelles. Microtubule depolymerization resulted coaggregation collapse filaments. The spatial distributions organelles compared filaments identical, indicating that attachment responsible organelle aggregation. Mitochondrial associations on other hand, retained, association stable regardless form In taxol-treated cells, taxol-stabilized bundles.