A statistical comparison of two culturing methods for enumerating sulfate-reducing bacteria

摘要: Abstract The addition of alkaline pyrogallol-soaked cotton wool plugs has been used by other workers to remove oxygen from the headspace gas in culture tubes for growth sulfate-reducing bacteria (SRB). This study compares enumeration fo SRB using most probable number (MPN) method with and without pyrogallol plugs. In both cases, liquid medium contained two iron finishing nails which help reduce redox potential medium. For each 25 samples oil fields, cooling water systems natural environments, time-consuming screw cap yielded virtually same MPN values as Kaput® closures on tubes. However, plug method, requires approximately 10-fold more technician time, was superior pure cases where greatly outnumber heterotrophs.