Characterization of monocyte differentiation-inducing (MDI) factors derived from human fetal membrane chorion cells undergoing apoptosis after influenza virus infection.

摘要: Abstract Influenza virus infection during pregnancy has been implicated as one of cause premature delivery, abortion and stillbirth. We have reported that cultured human fetal membrane chorion cells undergoing apoptosis by influenza secrete unidentified heat-stable monocyte differentiation-inducing (MDI) factors. In this study, cellular, biological immunochemical characteristics MDI factors were investigated using monocytic leukemia THP-1 nitroblue tetrazolium reduction cell adhesion assays. The treatment with culture supernatants from the virus-infected induced ability, which was inhibited addition superoxide dismutase diphenyleneiodonium chloride, an inhibitor for reduced nicotinamide adenine dinucleotide phosphate oxidase. phenomenon also observed in peripheral blood monocytes histiocytic U937 cells, but not promyelocytic HL-60 cells. induction abilities closely correlated concentrations interleukin-6 protein supernatants. These to approximately 60% antibodies against interleukin-6, or α-chain (gp80) β-chain (gp130) IL-6 receptor. increased amniochorion tissue cultures after infection. results indicate cell-derived is partly responsible differentiation macrophages capable generating anion. It possible these pathways represent part mechanism birth complications associated intrauterine pregnancy.