Inactivation of iron-sulfur cluster biogenesis regulator SufR in Synechocystis sp. PCC 6803 induces unique iron-dependent protein-level responses

摘要: Abstract Background Iron-sulfur (Fe-S) clusters are protein-bound cofactors associated with cellular electron transport and redox sensing, multiple specific functions in oxygen-evolving photosynthetic cyanobacteria. The aim here was to elucidate protein-level effects of the transcriptional repressor SufR involved regulation Fe-S cluster biogenesis cyanobacterium Synechocystis sp. PCC 6803. Methods approach quantitate 94 pre-selected target proteins various metabolic using SRM Synechocystis. evaluation conducted response sufR deletion under different iron conditions, complemented EPR analysis on functionality photosystems I II as well RT-qPCR verify also transcript level. Results results both protein levels show that acts not only a suf operon when is available but has other direct indirect cell, including maintenance expression pyruvate:ferredoxin oxidoreductase NifJ sufficient conditions. Furthermore, imply absence repressed by some additional regulatory mechanism independent SufR. Conclusions study demonstrates metabolism stringently regulated, complex interactions primary photosynthesis central carbon metabolism. General significance provides new insight into via operon, wide-ranging changes