Global Transcriptional Profiles of the Copper Responses in the Cyanobacterium Synechocystis sp. PCC 6803

摘要: Copper is an essential element involved in fundamental processes like respiration and photosynthesis. However, it becomes toxic at high concentration, which has forced organisms to control its cellular concentration. We have recently described a copper resistance system the cyanobacterium Synechocystis sp. PCC 6803, mediated by two-component system, CopRS, RND metal transport CopBAC protein of unknown function, CopM. Here, we report transcriptional responses additions non-toxic (0.3 µM) concentrations (3 wild type sensitive copR mutant strain. While 0.3 µM slightly stimulated metabolism promoted exchange between cytochrome c6 plastocyanin as soluble electron carriers, addition 3 catalyzed formation ROS, led general stress response induced expression Fe-S cluster biogenesis genes. According this, double strain copRsufR, expresses constitutively sufBCDS operon, tolerated higher concentration than strain, suggesting that clusters are direct targets toxicity Synechocystis. In also demonstrated InrS, nickel binding repressor belong CsoR family factor, was heavy homeostasis, including copper, Finally, global gene analysis suggested CopRS only controls copMRS copBAC operons copper.