Genome-wide analysis of transcription and photosynthesis inhibition in the harmful dinoflagellate Prorocentrum minimum in response to the biocide copper sulfate

摘要: Copper is an essential trace metal for organisms; however, excess copper may damage cellular processes. Their efficiency and physiological effects of biocides have been well documented; molecular transcriptome responses to are insufficiently studied. In the present study, a 6.0K oligonucleotide chip was developed investigate harmful dinoflagellate Prorocentrum minimum sulfate (CuSO4) treatment. The results revealed that 515 genes (approximately 8.6%) responded CuSO4, defined as being within 2-fold change. Further, KEGG pathway analysis showed differentially expressed (DEGs) were involved in ribosomal function, RNA transport, carbon metabolism, biosynthesis amino acids, photosystem maintenance, other Among DEGs, 49 related chloroplasts mitochondria. Furthermore, RAS signaling pathway, MAPK transport pathways identified. An additional experiment photosynthesis decreased considerably, reactive oxygen species (ROS) production increased P. after CuSO4 exposure. These suggest caused oxidative stress minimum, affecting ribosome mitochondria, severely damaged photosystem. potentially lead cell death, although has complex signal transduction process combat toxicity.