Diurnal Regulation of Cellular Processes in the Cyanobacterium Synechocystis sp. Strain PCC 6803: Insights from Transcriptomic, Fluxomic, and Physiological Analyses

摘要: ABSTRACT Synechocystis sp. strain PCC 6803 is the most widely studied model cyanobacterium, with a well-developed omics level knowledgebase. Like lifestyles of other cyanobacteria, that tuned to diurnal changes in light intensity. In this study, we analyzed expression patterns all genes cyanobacterium over two consecutive periods. Using stringent criteria, determined transcript levels nearly 40% show robust oscillating behavior, majority transcripts being upregulated during early period. Such corresponded wide array cellular processes, such as harvesting, photosynthetic and dark reactions, central carbon metabolism. contrast, membrane transporters for transition metals involved electron transport chain (e.g., iron, manganese, copper) were significantly late Thus, pattern global gene led development distinct transcriptional networks coregulated oscillatory genes. These help describe how regulates its metabolism toward end period anticipation efficient photosynthesis Furthermore, silico flux prediction important processes experimental measurements ATP, NADP(H), glycogen showed behavior influences metabolic characteristics. particular, NADPH/NADP + strong correlation whose peaks light. We conclude ratio key endogenous determinant cyanobacterium. IMPORTANCE Cyanobacteria are microbes use energy from sunlight CO 2 feedstock. Certain cyanobacterial strains amenable facile genetic manipulation, thus enabling synthetic biology engineering applications. developed chassis sustainable production food, feed, fuel. To end, holistic knowledge physiology under cycle warranted. report, genomewide analysis 6803, sheds on coordination found that, addition light, redox NADP(H) an regulator entrainment 6803.