Pericytes as collagen-producing cells in excessive dermal scarring.

摘要: Immunohistochemistry and image analysis were performed on sections from excessive dermal scar formation to investigate the potential of pericytes differentiate into collagen-producing cells. Expression prolyl-4-hydroxylase beta-subunit (P-4-H) was used as a marker for collagen synthesis distribution this protein identical procollagen type I C-propeptide similar cells expressing pro alpha 1(I) mRNA. Double immunofluorescence stainings using combinations monoclonal antibodies specific activated in vivo (high molecular weight-melanoma associated antigen (HMW-MAA)), P-4-H, smooth muscle alpha-actin (SMA), endothelial (PAL-E), platelet-derived growth factor (PDGF) beta-receptor, integrin 5 subunit performed. Stained analyzed by computerized allowing quantification degree colocalization between pairs antigens same tissue section. Four different subpopulations HMW-MAA discerned. The first subpopulation corresponded intramural pericytes, juxtapositioned endothelium, that expressed HMW-MAA, SMA, PDGF but not P-4-H. second partly dissociated microvascular wall exhibited expression except decrease SMA. Cells third located perivascular space subunit, beta-receptor and, albeit less pronounced, fourth no SMA strong Moreover, an vitro derived isolated fragments human dermis revealed pattern phenotypical change. Taken together data suggest population migrate develop collagen-synthesizing fibroblasts during fibrosis.