Filamentous phages as cloning vectors.

摘要: The virtue of Ff vectors goes beyond the fact that they deliver a single strand in convenient package for sequencing and oligonucleotide-directed mutagenesis. Of all common use are easiest to propagate process. Their genomes can be easily manipulated, knowledge acquired over quarter century basic research makes their behavior reasonably predictable. For this reason I have emphasized general properties phage review dealt at some length with applications still not fully developed, hope will inspire readers continue tradition imaginative exploitation unique class viruses.