A helper phage to improve single-chain antibody presentation in phage display

摘要: We show here that the number of single-chain antibody fragments (scFv) presented on filamentous phage particles generated with display phagemids can be increased by more than two orders magnitude using a newly developed helper (hyperphage). Hyperphage have wild-type pIII phenotype and are therefore able to infect F(+) Escherichia coli cells high efficiency; however, their lack functional gene means phagemid-encoded pIII-antibody fusion is sole source in assembly. This results an considerable increase fraction carrying fragment surface. Antigen-binding activity was about 400-fold enforced oligovalent every particle. When used for packaging universal human scFv library, hyperphage improved specific enrichment factor obtained when panning tetanus toxin. After rounds, 50% were found bind antigen, compared 3% conventional M13KO7 used. Thus, particularly useful stoichiometric situations, there little chance single will locate desired antigen.