MiRNA-200a induce cell apoptosis in renal cell carcinoma by directly targeting SIRT1
作者： Hao Fu , Wenke Song , Xuancai Chen , Tao Guo , Bin Duan
DOI: 10.1007/S11010-017-3102-1
关键词:
摘要: Accumulating evidence indicates that microRNAs are implicated in tumor initiation and progression through negatively regulating oncogenes or suppressor genes. In the present study, we report expression of miR-200a was significantly lower renal cell carcinoma (RCC) specimens RCC lines. Restoration suppressed growth, arrested cycle progression, promoted apoptosis We next used qRT-PCR array technology to identify Sirtuin 1 (SIRT1) as one downregulated proteins during overexpression 786-O cells. Following a further assay by luciferase reporter system, SIRT1 validated direct target miR-200a. Moreover, siRNA-mediated knockdown could partially phenocopy effects overexpression. contrast, truncated (without an endogenous 3'-UTR) rescue effect on cells, which suggested 3'-UTR is targeted specifically. These observations provide for critical tumor-suppressive role addition identifying novel regulatory mechanism, may contribute upregulation RCC.
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