Nuclear retention of the lncRNA SNHG1 by doxorubicin attenuates hnRNPC–p53 protein interactions

作者: Yuan Shen , Shanshan Liu , Jiao Fan , Yinghua Jin , Baolei Tian

DOI: 10.15252/EMBR.201643139

关键词:

摘要: Abstract The protein p53 plays a crucial role in the regulation of cellular responses to diverse stresses. Thus, major priority cell biology is define mechanisms that regulate activity response stresses or maintain it at basal levels under normal conditions. Moreover, further investigation required establish whether RNA participates regulating p539s interaction with other proteins. Here, by conducting systematic experiments, we discovered interactor—hnRNPC—that directly binds p53, destabilizes it, and prevents its activation Upon doxorubicin treatment, lncRNA SNHG1 retained nucleus through binding nucleolin competes for hnRNPC binding, which upregulates promotes p53‐dependent apoptosis impairing activity. Our results indicate balance between regulates upon change subcellular localization specific circumstances biologically significant.

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