Interferon-gamma inhibits proliferation, differentiation, and creatine kinase activity of cultured human muscle cells. II. A possible role in myositis.

摘要: Objective To investigate the effects of human interferon-gamma (IFN-gamma) on cultured skeletal muscle cells. Methods Muscle cell cultures were treated with various concentrations recombinant IFN-gamma, and proliferation, creatine kinase synthesis cytotoxicity analyzed. Results Treatment IFN-gamma resulted in significant inhibition myoblasts growth, fusion into multinucleated myotubes. inhibited if applied before, but not after, begin to differentiate The effect was dose dependent observed at a concentration as low 10 U/ml. Despite these cytostatic effects, cytotoxic cells even very high (10,000 U/ml) doses. Conclusion inhibits proliferation differentiation vitro. These findings suggest that T lymphokine, may inhibit regeneration repair injured fibers myositis.