Activation of p38 mitogen‐activated protein kinase is critical step for acquisition of effector function in cytokine‐activated T cells, but acts as a negative regulator in T cells activated through the T‐cell receptor

摘要: Summary Peripheral blood CD4+ CD45RO+ T cells activated in vitro are able to induce expression of tumour necrosis factor-α (TNF-α) monocytes via a contact-dependent mechanism. Activation is achieved either with interleukin-2 (IL-2)/IL-6/TNF-α over an 8-day period or cross-linking CD3 using anti-CD3 antibody for 48 hr. In this paper, we show that the p38 mitogen-activated protein kinase (MAPK) signalling pathway played different roles generation effector function these two types cells. cells, MAPK negative regulator induced cell proliferation and has no significant effect on acquisition (induction monocyte-derived TNF-α) production T-cell cytokines. contrast, required cytokine-induced promotes cytokine production.