Kosmotoga pacifica sp. nov., a thermophilic chemoorganoheterotrophic bacterium isolated from an East Pacific hydrothermal sediment

摘要: A novel strictly anaerobic thermophilic heterotrophic bacterium, strain SLHLJ1(T), was isolated from a Pacific hydrothermal sediment. Cells were Gram-negative coccobacilli (approximately 1.0 × 0.6 μm) with toga. It grew at temperatures between 33 and 78 °C (optimum 70 °C). Elemental sulphur L-cystine stimulated its growth. contained C16:0, C16:1 ω11c, C18:0 C18:1 ω9c as major fatty acids (>5%), 3 phospholipids 2 glycolipids polar lipids. Its DNA G+C content 43.7 mol%. Phylogenetic analyses based on 16S rRNA gene sequences placed SLHLJ1(T) within the family Thermotogaceae. The isolate most closely related to Kosmotoga arenicorallina (97.93 % sequence similarity), K. olearia (92.43%) shengliensis (92.17 %). On basis of phenotypic, chemotaxonomic phylogenetic comparisons closest relatives, we propose assignment species genus Kosmotoga. name pacifica sp. nov. is proposed (=DSM 26965(T) = JCM 19180(T) UBOCC 3254(T)) type species.