Identification and sequence analysis of the Bacillus subtilis W23 xylR gene and xyl operator.

摘要: The xyl operator of Bacillus subtilis W23 was identified by deletion analysis the regulatory region as a 25-base-pair (bp) sequence located 10 bp downstream from promoter. outer exhibit perfect palindromic symmetry, while 5 central are nonpalindromic. It demonstrated that penultimate base pair near end this is important for repressor binding. location xylR gene encoding determined its ability to mediate xylose-dependent repression xyl-cat fusion on multicopy plasmid. nucleotide 1,355 DNA analyzed and contains an open reading frame with coding capacity 384 amino acids leading protein calculated molecular weight 42,270. A mutant in showed no fusion. preceded suitable ribosome-binding uses GTG start codon TAA stop codon. relationship these results corresponding data obtained B. 168 discussed.