Cloning and Characterization of Transcription of the xylAB Operon in Thermoanaerobacter ethanolicus
作者： Milutin Erbeznik , Karl A. Dawson , Herbert J. Strobel
DOI: 10.1128/JB.180.5.1103-1109.1998
关键词:
摘要: The genes encoding xylose isomerase (xylA) and xylulose kinase (xylB) from the thermophilic anaerobe Thermoanaerobacter ethanolicus were found to constitute an operon with transcription initiation site 169 nucleotides upstream previously assigned (K. Dekker, H. Yamagata, K. Sakaguchi, S. Udaka, Agric. Biol. Chem. 55:221–227, 1991) promoter region. bicistronic xylAB mRNA was processed by cleavage within 5′-terminal portion of XylB-coding sequence. Transcription induced in presence xylose, and, unlike all other xylose-utilizing bacteria studied, not repressed glucose. existence putative xyl operator sequences suggested that utilization is controlled a repressor-operator mechanism. T. xylB gene coded for 500-amino-acid-residue protein deduced amino acid sequence highly homologous those XylBs. This first report nucleotide anaerobic bacterium.
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