N-glycoprotein profiling of lung adenocarcinoma pleural effusions by shotgun proteomics.

摘要: BACKGROUND Malignant pleural effusion of advanced lung adenocarcinoma may be a valid source for detection biomarkers, such as N-glycosylated proteins (N-GP), because tumor cells grow during weeks in this liquid. The authors aimed creation N-GP profiles from routine cytology specimens to detect relevant biomarkers. METHODS Hundred microliters malignant effusions 5 patients with and nonmalignant controls were used triplicate capture by solid-phase extraction. After trypsin digest PNGase F release, liquid chromatography separation connected online tandem mass spectrometer was performed chromatography/tandem spectrometry (LC/MS/MS). RESULTS In the total 10 samples, 170 278 nonredundant detected probabilities ≥.9 ≥.5, respectively. specificity N-glycomotif 88% at P ≥ .9. Penetration into moderate low protein concentration range (μg-ng/mL) occurred, several associated progression or metastasis identified, including CA-125, CD44, CD166, lysosome-associated membrane glycoprotein 2 (LAMP-2), multimerin 2, periostin. MS identifications correlated corresponding immunoreactivity either fluid tissue. CONCLUSIONS In conclusion, reduction sample complexity capturing allows μg ng/mL range. Pleural is useful biomarker research cancer. Cancer (Cancer Cytopathol) 2008. © 2008 American Society.