Copper modulates sex-specific fructose hepatoxicity in nonalcoholic fatty liver disease (NALFD) Wistar rat models.

摘要: This study aimed to characterize the impact of dietary copper on biochemical and hepatic metabolite changes associated with fructose toxicity in a Wistar rat model fructose-induced liver disease. Twenty-four male 24 female, 6-week-old, Wister rats were separated into four experimental treatment groups (6 males 6 females per group), as follows: (1) control diet: containing no adequate (i.e., CuA/0% Fruct); (2) diet regimen identical supplemented 30% w/v animals' drinking water (CuA/30% (3) but deficient content (CuD/0% Fruct) (4) (CuD/30% Fruct). The animals fed regimens for 5 weeks, followed by euthanization assessment histology, elemental profiles identification quantitation metabolites. Results from 1H nuclear magnetic resonance metabolomics revealed mechanistic insights modulation hepatotoxicity through distinct metabolic phenotypes that highly correlated sex. also identified previously unknown sex-specific responses both supplementation restricted intake, while presence promoted most pronounced changes.