Apoptosis and neutrophils in the regulation of Ph-positive myeloid cell proliferation and differentiation ex vivo

摘要: Ex vivo proliferation and differentiation of Philadelphia chromosome-positive (Ph+) human myeloid cells (Ph+ cells) from chronic leukemia (CML) proceed via alternation stages cell neutrophil maturation. To regulate them, apoptosis is alternately blocked or induced with the help neutrophils expression bcr/abl, bax, bcl2. The regulation in main types Ph+ depends on (1) (2) maturation may follow two pathways. One consists alternating blockages inductions initial subsequent under as (2)-(1)-(2) has not been described yet. Neutrophil accumulation blocks apoptosis. As are depleted, again. Its block accelerates a new neutrophils, which followed by regular death induction way optimizes efficiency (P/D index) proliferation, allowing cycle to be completed. In another way, starts (1)-(2)-(1) at lower content) leads resistant decrease maximal level factor 3–8 compared alternation. A stable observed prolonged maturation, leading an blasts myelocytes elevated bcr/abl bcl2 > bax. associated CML progression lines. Cells first pathway apoptotic chronic-phase CML. cultivation individual patients was assumed provide for more exact diagnosis phase optimizing treatment.