Gene expression upon proliferation and differentiation of hematopoietic cells with Ph chromosome ex vivo

摘要: The genes p53, mdm2, p21, c-myc, bcr/abl, bcr, bcl2, bax, and gapdh participate in the regulation of cell proliferation differentiation, apoptosis distribution for cycle ex vivo Ph + cells chronic myeloid leukemia containing chromosome bcr/abl oncogene. Expression these correlates with differentiation by alternating maturation stages three main Ph+cell types that occur under leukemia. overexpress active proliferating S+ G2/M phases when are coincident stage. decreases to a considerable level alter- nation whenever expression is greatly diminished un- der significant neutrophil accumulation especially repeated alternation stages. In course maturation, gene levels decrease range > actin bcr/abl,p21 p53 bcl2 bax. neutrophils lower than those myelocytes an order magnitude prolonged Bcr/abl inhibited; however it enhanced 2-3 times stage myelocyte accumulation. Minimal observed overexpres- sion as well maximum at S phases. overexpression low mdm2 genes. high P/D efficiency index (5-20), bcr> gapdh>bcr/abl, decreased p21 р53, c-myc blocking apoptosis, accumulation,